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ISOLATION AND CHARACTERISATION OF MULTI DRUG-RESISTANT BACTERIA FROM SURGICAL EQUIPMENTS IN GENERAL HOSPITAL


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  • U.M. OYEDUM Department of Microbiology, School of Life Sciences, Federal University of Technology, Minna
  • O.S. OLATUNJI Department of Microbiology, School of Life Sciences, Federal University of Technology, Minna
  • H. ABU Department of Microbiology, School of Life Sciences, Federal University of Technology, Minna
Keywords: Bacteria, Hospitals, Multi drug-resistant bacteria, Surgical equipments, Theatres

Abstract

The predominance of acquired resistance, especially multidrug-resistance among bacterial isolates is fast becoming a major menace associated with health-care equipments and facilities. Samples were collected from four (4) surfaces of various surgical equipments such as operating tables, trolleys, beds and drip stands in the surgical theatre in General Hospital Minna. The samples were collected using sterile swab sticks dipped in normal saline and were transported to the Microbiology laboratory at Federal University of Technology Minna for further analysis. Samples were inoculated on various culture media by streaking. Bacterial isolates were identified through Gram staining and other biochemical tests. The identified bacterial isolates were subjected to an antibiogram using the disc diffusion method. The result revealed that all the surgical equipments studied had a high bacterial count (0.02x102) except the drip stand which had a low bacterial count (0.01x102). Salmonella sp. had the highest frequency of occurrence (42.8%) while Enterobacter sp. and Klebsiella sp. had the least frequency of occurrence (14.3%). The study also revealed that multidrug- resistant bacteria were associated with the various equipments in the surgical theatre. Therefore, there is a need for continuous surveillance of these equipments to avoid the accumulation of multidrug- resistant (MDR) bacteria which could bring about nosocomial infections that could be difficult to curtail and control.


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Published
2023-06-29
How to Cite
OYEDUM, U., OLATUNJI, O., & ABU, H. (2023). ISOLATION AND CHARACTERISATION OF MULTI DRUG-RESISTANT BACTERIA FROM SURGICAL EQUIPMENTS IN GENERAL HOSPITAL . African Journal of Health, Safety and Environment, 4(1), 59-64. https://doi.org/10.52417/ajhse.v4i1.393