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ASSESSMENT OF RENAL FUNCTION IN DIABETIC WISTAR RATS TREATED WITH ETHANOL EXTRACT OF Cucumis sativus FRUIT.


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  • O.D. ABU Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria.
  • E .P. AWHIN Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Delta State University, Abraka, Delta State, Nigeria.
  • H.E. IYARE Department of Science Laboratory Technology, Shaka Polytechnic, Benin City, Nigeria.
Keywords: Electrolytes, Histology, Medicinal plant, Renal toxicity, Urease.

Abstract

As a fast-growing metabolic disorder diabetes mellitus is one of the leading causes of death worldwide. Nephropathy (damage to kidney leading to renal failure) is a microvascular complication of the disease. The aim of the present study was to assess renal function in diabetic rats treated with ethanol extract of Cucumis sativus fruit. Male Wistar rats (n = 25, mean weight = 215 ± 15 g) were randomly assigned to five groups (5 rats per group): control, diabetic, metformin, 200 mg/kg body weight (bwt) extract and 300 mg/kg bwt extract groups. Diabetes mellitus was induced in the rats via intraperitoneal injection of streptozotocin (STZ) at a dose of 50 mg/kg bwt. The diabetic rats were then treated for 21 days with metformin (50 mg/kg bwt) or the extract at doses of 200 and 300 mg/kg bwt, respectively, leaving the diabetic group untreated. The results showed that induction of diabetes mellitus using STZ significantly increased plasma urease activity, and urea and chloride concentrations, but it reduced the weight of rat kidney and concentrations of sodium, potassium and bicarbonate ions significantly (p < 0.05). However, treatment of the diabetic rats with the extract markedly reduced plasma urease, and urea and chloride ion concentrations, while increasing kidney weight, organ/body weight ratio as well as concentrations of sodium, potassium and bicarbonate ions (p < 0.05). The effect of the extract on potassium and chloride ions was dose-dependent. These results indicate that ethanol extract from the medicinal plant C. sativus fruit can ameliorate kidney dysfunction caused by STZ-induced diabetes mellitus.


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Published
2023-06-29
How to Cite
ABU, O., AWHIN, E. .P., & IYARE, H. (2023). ASSESSMENT OF RENAL FUNCTION IN DIABETIC WISTAR RATS TREATED WITH ETHANOL EXTRACT OF Cucumis sativus FRUIT. African Journal of Health, Safety and Environment, 4(1), 101-107. https://doi.org/10.52417/ajhse.v4i1.403